كتاب  Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in short-term erythrocyte culturesكتب الطب

كتاب Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in short-term erythrocyte cultures

Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in shortterm erythrocyte cultures من كتب طب بيطرى Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in short-term erythrocyte cultures J. F. COETZEE* M. D. APLEY*, K. M. KOCAN & D. E. JONES à * Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA; Department of Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, USA; à Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA Coetzee, J. F., Apley, M. D., Kocan, K. M., Jones, D. E. Flow cytometric evalu- ation of selected antimicrobial efficacy for clearance of Anaplasma marginale in short-term erythrocyte cultures. J. vet. Pharmacol. Therap. 29, 173–183. The tick-borne rickettsia, Anaplasma marginale , causes the economically important cattle disease anaplasmosis. Once infected, cattle remain lifelong carriers. Herein, we used flow cytometry to test the efficacy of three antimicrobials; oxytetracycline, imidocarb and enrofloxacin against Virginia (VGN) or Oklahoma (OK) A. marginale isolates in short-term erythrocyte cultures. Parasite viability was assessed using the vital dye hydroethidine (HE), which is detectable when living organisms convert HE to ethidium bromide. Viability of A. marginale in selected cultures was determined by subinoculation into susceptible calves. Data were analyzed by MANOVA, Tukey-Kramer honest significant difference and Wilcoxon rank sum tests. Receiver operating characteristic (ROC) analysis was used to correlate results with culture infectivity. Enrofloxacin inhibited A. marginale in a dose dependent manner. Surprisingly, higher concentrations of imidocarb were less effective than lower concentrations against A. marginale with significant differences ( P < 0.05) observed between the two isolates. Oxytetracycline was the least active drug tested. Cultures infected with the OK isolate exposed to 4.0 l g/mL enrofloxacin and those of the VGN and OK isolates exposed to 1.0 l g/mL imidocarb were sterilized. This is the first in vitro study demonstrating the efficacy of enrofloxacin against A. marginale . Furthermore, these data indicate that flow cytometry is a useful assay for screening antimicrobials against A. marginale . (Paper received 16 November 2005; accepted for publication 17 February 2006) Hans Coetzee, Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506–5601, USA. E-mail: [email protected] vet.ksu.ed
-
من كتب طب بيطرى - مكتبة كتب الطب.

وصف الكتاب : Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in shortterm erythrocyte cultures من كتب طب بيطرى

Flow cytometric evaluation of selected antimicrobial efficacy for clearance
of
Anaplasma marginale
in short-term erythrocyte cultures
J. F. COETZEE*
M. D. APLEY*,
K. M. KOCAN
&
D. E. JONES
à
*
Department of Veterinary Diagnostic
and Production Animal Medicine,
College of Veterinary Medicine,
Iowa State University,
Ames, IA, USA;
Department of
Pathobiology, Center for Veterinary Health
Sciences, Oklahoma State University,
Stillwater, OK, USA;
à
Department of
Veterinary Pathology, College of Veterinary
Medicine, Iowa State University, Ames,
IA, USA
Coetzee, J. F., Apley, M. D., Kocan, K. M., Jones, D. E. Flow cytometric evalu-
ation of selected antimicrobial efficacy for clearance of
Anaplasma marginale
in
short-term erythrocyte cultures.
J. vet. Pharmacol. Therap.
29,
173–183.
The tick-borne rickettsia,
Anaplasma marginale
, causes the economically
important cattle disease anaplasmosis. Once infected, cattle remain lifelong
carriers. Herein, we used flow cytometry to test the efficacy of three
antimicrobials; oxytetracycline, imidocarb and enrofloxacin against Virginia
(VGN) or Oklahoma (OK)
A. marginale
isolates in short-term erythrocyte
cultures. Parasite viability was assessed using the vital dye hydroethidine (HE),
which is detectable when living organisms convert HE to ethidium bromide.
Viability of
A. marginale
in selected cultures was determined by subinoculation
into susceptible calves. Data were analyzed by MANOVA, Tukey-Kramer honest
significant difference and Wilcoxon rank sum tests. Receiver operating
characteristic (ROC) analysis was used to correlate results with culture
infectivity. Enrofloxacin inhibited
A. marginale
in a dose dependent manner.
Surprisingly, higher concentrations of imidocarb were less effective than lower
concentrations against
A. marginale
with significant differences (
P
< 0.05)
observed between the two isolates. Oxytetracycline was the least active drug
tested. Cultures infected with the OK isolate exposed to 4.0
l
g/mL enrofloxacin
and those of the VGN and OK isolates exposed to 1.0
l
g/mL imidocarb were
sterilized. This is the first
in vitro
study demonstrating the efficacy of
enrofloxacin against
A. marginale
. Furthermore, these data indicate that flow
cytometry is a useful assay for screening antimicrobials against
A. marginale
.
(Paper received 16 November 2005; accepted for publication 17 February
2006)
Hans Coetzee, Department of Clinical Sciences, College of Veterinary Medicine,
Kansas State University, Manhattan, KS 66506–5601, USA. E-mail: [email protected]
vet.ksu.ed

عدد مرات التحميل : 6255 مرّة / مرات.
تم اضافته في : السبت , 26 مارس 2016م.
حجم الكتاب عند التحميل : 252.1 كيلوبايت .

ولتسجيل ملاحظاتك ورأيك حول الكتاب يمكنك المشاركه في التعليقات من هنا:

Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in short-term erythrocyte cultures من كتب طب بيطرى

Coetzee, J. F., Apley, M. D., Kocan, K. M., Jones, D. E. Flow cytometric evalu-
ation of selected antimicrobial efficacy for clearance of
Anaplasma  marginale
in
short-term erythrocyte cultures.
J.  vet.  Pharmacol.  Therap.
29,
173–183.
The tick-borne rickettsia,
Anaplasma    marginale
, causes the economically
important cattle disease anaplasmosis. Once infected, cattle remain lifelong
carriers. Herein, we used flow cytometry to test the efficacy of three
antimicrobials; oxytetracycline, imidocarb and enrofloxacin against Virginia
(VGN) or Oklahoma (OK)
A.   marginale
isolates in short-term erythrocyte
cultures. Parasite viability was assessed using the vital dye hydroethidine (HE),
which is detectable when living organisms convert HE to ethidium bromide.
Viability of
A.  marginale
in selected cultures was determined by subinoculation
into susceptible calves. Data were analyzed by MANOVA, Tukey-Kramer honest
significant difference and Wilcoxon rank sum tests. Receiver operating
characteristic (ROC) analysis was used to correlate results with culture
infectivity. Enrofloxacin inhibited
A.  marginale
in a dose dependent manner.
Surprisingly, higher concentrations of imidocarb were less effective than lower
concentrations against
A.   marginale
with significant differences (
P
< 0.05)
observed between the two isolates. Oxytetracycline was the least active drug
tested. Cultures infected with the OK isolate exposed to 4.0
l
g/mL enrofloxacin
and those of the VGN and OK isolates exposed to 1.0
l
g/mL imidocarb were
sterilized. This is the first
in   vitro
study demonstrating the efficacy of
enrofloxacin against
A.  marginale
. Furthermore, these data indicate that flow
cytometry is a useful assay for screening antimicrobials against
A.  marginale
.
(Paper received 16 November 2005; accepted for publication 17 February
2006)
Hans   Coetzee,   Department   of   Clinical   Sciences,   College   of   Veterinary   Medicine,
Kansas  State  University,  Manhattan,  KS  66506–5601,  USA.  E-mail:  [email protected]
vet.ksu.ed



نوع الكتاب : PDF.
اذا اعجبك الكتاب فضلاً اضغط على أعجبني
و يمكنك تحميله من هنا:

تحميل  Flow cytometric evaluation of selected antimicrobial efficacy for clearance of Anaplasma marginale in short-term erythrocyte cultures



كتب اخرى في كتب طب بيطرى

Bacteriological and epidemiological findings during examination of the uterine content of ewes with retention of fetal membranes PDF

قراءة و تحميل كتاب Bacteriological and epidemiological findings during examination of the uterine content of ewes with retention of fetal membranes PDF مجانا

Bovine mastitis caused by Mycoplasma bovis PDF

قراءة و تحميل كتاب Bovine mastitis caused by Mycoplasma bovis PDF مجانا

Epsiprantel PDF

قراءة و تحميل كتاب Epsiprantel PDF مجانا

Exogenous control of follicular wave emergence in cattle PDF

قراءة و تحميل كتاب Exogenous control of follicular wave emergence in cattle PDF مجانا

Enrofloxacin to Control Anaplasma marginale Infections PDF

قراءة و تحميل كتاب Enrofloxacin to Control Anaplasma marginale Infections PDF مجانا

Enrofloxacin pharmacokinetics in the European cuttlefish, Sepia officinalis, after a single i.v. injection and bath administration PDF

قراءة و تحميل كتاب Enrofloxacin pharmacokinetics in the European cuttlefish, Sepia officinalis, after a single i.v. injection and bath administration PDF مجانا

Enrofloxacin serum bioactivity in bottlenose dolphins, Tursiops truncatus, following oral administration of 5 mg per kg in whole fish PDF

قراءة و تحميل كتاب Enrofloxacin serum bioactivity in bottlenose dolphins, Tursiops truncatus, following oral administration of 5 mg per kg in whole fish PDF مجانا

Fallibility of faecal consistency as a criterion of success in the evaluation of oral fluid therapy for calf diarrhoea PDF

قراءة و تحميل كتاب Fallibility of faecal consistency as a criterion of success in the evaluation of oral fluid therapy for calf diarrhoea PDF مجانا

المزيد من كتب الطب في مكتبة كتب الطب , المزيد من كتب صيدلة في مكتبة كتب صيدلة , المزيد من الطب البشرى العام في مكتبة الطب البشرى العام , المزيد من كتب تمريض في مكتبة كتب تمريض , المزيد من كتب طب اسنان في مكتبة كتب طب اسنان , المزيد من كتب طب بيطرى في مكتبة كتب طب بيطرى , المزيد من الطب التكميلي - الطب البديل في مكتبة الطب التكميلي - الطب البديل , المزيد من كتب علاج طبيعى في مكتبة كتب علاج طبيعى
عرض كل كتب الطب ..
اقرأ المزيد في مكتبة كتب تقنية , اقرأ المزيد في مكتبة كتب إسلامية , اقرأ المزيد في مكتبة كتب الهندسة و التكنولوجيا , اقرأ المزيد في مكتبة كتب التنمية البشرية , اقرأ المزيد في مكتبة الكتب التعليمية , اقرأ المزيد في مكتبة القصص و الروايات و المجلات , اقرأ المزيد في مكتبة كتب التاريخ , اقرأ المزيد في مكتبة كتب الأطفال قصص و مجلات , اقرأ المزيد في مكتبة كتب تعلم اللغات , اقرأ المزيد في مكتبة الكتب و الموسوعات العامة , اقرأ المزيد في مكتبة كتب الطب , اقرأ المزيد في مكتبة كتب الأدب , اقرأ المزيد في مكتبة كتب الروايات الأجنبية والعالمية , اقرأ المزيد في مكتبة كتب علوم سياسية و قانونية , اقرأ المزيد في مكتبة كتب اللياقة البدنية والصحة العامة , اقرأ المزيد في مكتبة الكتب الغير مصنّفة , اقرأ المزيد في مكتبة كتب الطبخ و الديكور , اقرأ المزيد في مكتبة كتب المعاجم و اللغات , اقرأ المزيد في مكتبة كتب علوم عسكرية و قانون دولي
جميع مكتبات الكتب ..